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Validation of microwave-assisted sample extraction-high performance liquid chromatography (HPLC) method for quantitative analysis of hydrolysed amino acids in aquatic biological samples

Authors:

G.D.T.M. Jayasinghe,

LK
About G.D.T.M.

Analytical Chemistry Laboratory, National Aquatic Resources Research & Development Agency (NARA), Crow Island, Colombo-15, Sri Lanka

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B.K.K.K. Jinadasa

LK
About B.K.K.K.
Analytical Chemistry Laboratory, National Aquatic Resources Research & Development Agency (NARA), Crow Island, Colombo-15, Sri Lanka
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Abstract

A sensitive and widely applicable High-Performance Liquid Chromatography (HPLC) with fluorescence detector (FLD) method for determination hydrolysed amino acids in fish and aquatic products was developed and validated through study. The method involved a microwave-assisted sample extraction and protein hydrolysed with the hydrochloric acid and propionic acid. The separation was performed by Shim-pack HR-ODS column with a gradient mobile phase. The total separation time was 25 min per run. The method showed satisfactory linearity, repeatability, accuracy, and limit of detection for 21 amino acids with the 1-100 mg/L calibration range based on 10 µL injection. The fish, fish feed, canned fish and seaweeds samples were analysed successfully by using this developed method.
How to Cite: Jayasinghe, G.D.T.M. and Jinadasa, B.K.K.K., 2019. Validation of microwave-assisted sample extraction-high performance liquid chromatography (HPLC) method for quantitative analysis of hydrolysed amino acids in aquatic biological samples. Sri Lanka Journal of Aquatic Sciences, 24(1), pp.17–23.
Published on 15 Mar 2019.
Peer Reviewed

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